Figure 3
Global multi-method analysis with nonideal self-association binding models for the panel of mAbs. Simultaneous fit of SLS (A), DLS (B), and SV isotherm (C) with a model describing a nonideal monomer for mAb B (red), monomer–dimer self-association for mAb A (blue) and mAb E (cyan), monomer–dimer-tetramer-isoenthalpic self-association for mAb D (green), and monomer–dimer–tetramer self-association of mAb C (magenta, solid line). For comparison, best GMMA fit of mAb C data to a monomer–dimer model (dashed magenta line) and an isodesmic self-association model (dotted magenta line) are additionally shown. Best-fit parameter estimates underlying the models are in Table 2.
