Figure 4

Relationship between hydrodynamic nonideality and second virial coefficients. Shown are the pairs of k_S and B₂ obtained by GMMA for the panel of mAbs (squares, mAb B in red, mAb A in blue, mAb E in cyan, mAb D in green, and mAb C in magenta), and our previously measured values³³ for two VRC IgGs (triangles) and NISTmAb in different solution conditions (circles). Statistical fluid mechanics theory predicts a relationship k_S = (3.03 + 3.52[B₂/B_HS])v*^20,21. The joint linear regression of all IgG data (dashed line) leads to a k_S value at ‘theta’ conditions (where B₂ = 0) of 5.4 mL/g and a slope of 0.505, implying v* = 1.8 mL/g and B_HS = 12.4 mL/g.