Table 4 Best-fit binding parameters from the GMMA of static and dynamic light scattering only.

From: Global multi-method analysis of interaction parameters for reversibly self-associating macromolecules at high concentrations

mAb

Model

KD,glob

B2 (mL/g)

kD (mL/g)

kS,app(a) (mL/g)

B

1 + irrev. 2(b)

∞ (> 1.3 mM)

1.09 (0.06)

 − 4.1 (0.4)

6.3 (0.41)

A

1–2

470 µM (420–530)

2.1 (0.5)

 − 6.9 (1.0)

11.0 (1.4)

E

1–2… isoH

600 µM (530–660)

 − 6.3 (0.4)

 − 11.9 (0.7)

0.7 (1.1)

D

1–2 and 2–4–…iso

82 µM (76–86.3) and 9.0 mM (1.3–∞)

 − 0.0 (0.73)

 − 3.9 0.87)

3.9 (1.7)

C

1–2 and 2–4

16.0 µM (15.3–16.8) and 40.2 µM (36.8–44.1)

 − 4.7 (0.6)

 − 21.9 (0.8)

12.5 (1.4)

  1. Values in parentheses are confidence intervals estimated from Monte-Carlo analysis.
  2. (a)Apparent kS calculated as kS = 2B2 − kD.
  3. (b)Irreversible dimer with best-fit population of 9% was required for the best fit.
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