Figure 5
Relationship between miR-199a-3p expression levels and PTGS2 mRNA expression levels in synovium-derived, cultured MCs from RA and OA patients. (A) Comparison of miR-199a-3p expression levels in synovium-derived, cultured MCs obtained from OA (n = 24) and RA (n = 12) patients. The data are shown as the ratio of the miR-199a-3p level to the RNU48 level (× 10–3, mean ± SEM). Significance was determined using the Mann–Whitney U test. (B,C) Plots of expression level of miR-199a-3p/RNU48 vs. expression level of PTGS2/GAPDH in synovium-derived, cultured MCs obtained from OA (n = 24, B) and RA (n = 12, C) patients. Each point represents one donor. (D) Illustration of procedure for transduction of miRNA mimic to synovium-derived, cultured MCs obtained from RA patients and activation of the cells (E). Expression levels of miR-199a-3p mRNA in control miRNA mimic-transduced (Ctrl) and miR-199a-3p mimic-transduced synovium-derived, cultured MCs obtained from RA patients (miR-199a-3p). The expression level of miR-199a-3p in control miRNA mimic-transduced cells was designated as 1. The results are the mean ± SEM for three different experiments. (F) Expression levels of PTGS2 mRNA in control miRNA mimic-transduced (Ctrl), and miR199a-3p mimic-transduced synovium-derived, cultured MCs obtained from RA patients (miR-199a-3p) in response to 10 ng/mL of TNF-α for 2 h. The expression level of PTGS2 mRNA in control miRNA mimic-transduced cells was designated as 1. The results are the mean ± SEM for three different experiments. *indicates P < .05.
