Figure 3
From: Optical Tweezers with Integrated Multiplane Microscopy (OpTIMuM): a new tool for 3D microrheology
taken from the central plane images of a 3 µm diameter bead as it was moved through z, with steps of 50 nm. (b) Cross-sections taken through the centre of the image of the 3 µm bead in the central plane as the bead is moved through z. The red dashed lines correspond to the spacing of the different segmented planes. Contrast and brightness adjusted for clarity. (Matlab 2019b; MathWorks, Natick, MA, https://uk.mathworks.com/products/matlab.html) (c)The calculated z-position of the bead using the sharpest plane (z.S.P, blue), centre of sharpness (zC.S, red), rescaled centre of sharpness (zR.C.S, green) approaches to determine the z position versus stage position in z. Stage position is also plotted as a dashed black line as a comparison. (d) Residuals of the zR.C.S data shown in (c).
(a) Sharpness (orange) and summed intensity (blue) lines
