Figure 4
From: Optical Tweezers with Integrated Multiplane Microscopy (OpTIMuM): a new tool for 3D microrheology
(a) 3D scatter plot of the trajectory of a ~ 7 µm diameter bead confined in space by an optical trap (Matlab 2019b; MathWorks, Natick, MA, https://uk.mathworks.com/products/matlab.html). (b) Projections of the trajectory on the x–y and x–z planes. The bead trajectory is drawn from the image analysis of ~ 100,000 frames. (c), (d) and (e) show the x, y, and z position respectively of the bead with time over the length of the experiment. (f) The bead NMSD versus lag-time τ evaluated for each dimension. (g) The particle NPAF for each dimension plotted against a dimensionless lag-time τ*42, derived from the scatter plots shown in (a). The solid line is at NPAF = e-1.
