Figure 1
Palmitic acid but not DHA, inhibits insulin action and up-regulates TLR4. Human SH-Y5Y neuroblastoma cells were pretreated with palmitic acid, DHA or placebo during 4 h, then insulin-dependent Akt and ERK1/2 phosphorylation measured by Western blots using adequate antibodies, Control, Palmitic acid and DHA blots were performed in separate membranes, in each case the same membrane was blotted with different antobodies (panel A); TLR4 expression was measured by Western blot using anti-TLR4 antibodies and the expression was normalized to β tubilin, and band densities were quantified and expressed as ratio of TLR4/β-tubilin (panel B); TLR4 mRNA expression was determined by qRT-PCR normalized to GAPDH (panel C). Western blot and qRT-PCR data were presented as means ± SEM (n = 3), * and ** denoted significant differences vs control at p < 0.05 and p < 0.005, respectively.
