Table 1 Parameters and options of elution test stated in ISO 10993.
Parameter | Options | Citation |
|---|---|---|
Extraction volume | 125 mm2 · ml−1 Or 0.2 g · ml−1 | ISO 10993-12 |
The velocity of orbital shaking during extraction | Shaking required, but velocity not specified | ISO 10993-12 |
FBS concentration in extraction medium | 5%, 10% or without FBS | ISO 10993-5 Annex A |
Cultivation medium composition (type) | According to the cell type | ISO 10993-5 |
Time of extraction | 24 ha | ISO 10993-5 |
Cell type | e.g. L929 cells | ISO 10993-5 Annex C |
Mode of exposition | Cell layer or freshly suspended cells | ISO 10993-5 |
Incubation time | At least 24 h | ISO 10993-5 |
