Figure 2 | Scientific Reports

Figure 2

From: The impact of the co-registration technique and analysis methodology in comparison studies between advanced imaging modalities and whole-mount-histology reference in primary prostate cancer

Figure 2

Illustration of the two pathways for co-registration of histopathological slices with in-vivo imaging. (A) shows co-registration workflow via intermediate ex-vivo CT (1) and ex-vivo MRI (2) imaging. First row shows H&E stained whole mount prostatectomy specimen with intraprostatic tumor lesions marked in blue. Second row shows intermediate GTV-Histo 1 and GTV-Histo 2 on ex-vivo CT (1) and ex-vivo MRI (2) in yellow (created in MITK Workbench 2015.5.2). Correct localisation of specimen slices was based on the 4 mm grid attached to the side walls of the localizer as well as anatomical landmarks. Third row shows GTV-Histo 1 (green) and GTV-Histo 2 (orange) after final co-registration with in-vivo CT (1) and in-vivo MRI (2). Additionally GTV-PET (red) in diagnostic [18F]PSMA-1007-PET/CT and GTV-MRI (purple) in diagnostic MRI (DWI ADC map) are shown. Fourth row shows GTV-Histo 1 (green), GTV-PET (red) and GTV-MRI (purple) in in-vivo CT (1) and in-vivo MRI (2) after final co-registration. (B). After manual co-registration of in-vivo CT and in-vivo MRI allowing non-rigid deformation, GTV-Histo 1 (green), GTV-Histo 2 (orange), GTV-MRI (purple) and GTV-PET (red) were superimposed on the corresponding in-vivo-CT image.

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