Figure 1

C. elegans consumption of glucose fed bacteria. (a) Experimental protocol used for OP50 E. coli processing of glucose and C. elegans assays. (b) Effect of 0.4% glucose supplementation on E. coli colony forming units over time (P = 0.002 at day 3). (c) Detection of Carboxymethyl-lysine (CML), an AGE product, by ELISA Assay performed on control and 0.4% glucose fed E. coli. (d) Lifespan of wild type C. elegans treated with 0% control and 0.4% glucose fed OP50 E. coli; Statistics in Supplementary Table S1, S2. (e) Healthspan—Movement in liquid/swimming/thrashing of C. elegans consuming control or 0.4% glucose fed E. coli, N = 232. (f) Oxidative stress resistance of wild type C. elegans consuming 0% control and or 0.4% glucose fed E. coli for 6 days, measured by mean survival on paraquat, N = 328. (g) Heat stress resistance (mean survival at 37 °C) of wild type C. elegans consuming 0% control and 0.4% glucose fed E. coli for 6 days, N = 366. Statistical analysis of histograms compared C. elegans consuming 0% control with C. elegans consuming 0.4% glucose fed E. coli at the same time point using an unpaired two-tailed t test with GraphPad Prism 8.0 (https://www.graphpad.com). Symbols as follows: (ns = not significant, *P ≤ 0.05, **P ≤ 0.005, ***P ≤ 0.001). Data shown is a compilation from at least 3 biological replicates.