Figure 3

Effects of bacterial metabolism of glucose on host C. elegans gene expression. (a) Fluorescent imaging of transgenic C. elegans gst-4::gfp, sod-3::gfp, or glod-4::gfp, after 6 days consuming either 0% control, 0.4% glucose, 0.4% 2-deoxy-glucose (2-DG), 50 mM carnosine, or 0.4% glucose + 50 mM carnosine fed E. coli for 6 days. See methods for complete genotype of transgenic C. elegans. (b) Fluorescence quantification of animals in (a): transgenic C. elegans gst-4::gfp, sod-3::gfp, or glod-4::gfp, consuming either 0% control, 0.4% glucose, 0.4% 2-deoxy-glucose (2-DG), 50 mM carnosine, or 0.4% glucose + 50 mM carnosine, fed E. coli for 6 days, N = 336. (c) RTqPCR of wild type C. elegans consuming either 0% control or 0.4% glucose fed E. coli for 6 days. Statistical analysis of histograms compared C. elegans consuming 0% control with C. elegans consuming 0.4% glucose fed E. coli at the same time point using an unpaired two-tailed t test with GraphPad Prism 8.0 (https://www.graphpad.com). Symbols as follows: (ns = not significant, *P ≤ 0.05, **P ≤ 0.005, ***P ≤ 0.001). Data shown is a compilation from at least 3 biological replicates.