Figure 1

Pathology of the sporadic MPNST from which the 2XSB cell line was derived. (a) A representative hematoxylin and eosin (H&E) stained image (×40) displaying the densely packed, poorly differentiated cells composing this tumor. (b,c) High power (×60) H&E stained images highlighting high grade pathologic features such as mitotic figures (arrows, b) and necrosis (asterisk, c). (d–f) Representative immunohistochemical staining images of Schwann cell markers in formalin-fixed paraffin-embedded (FFPE) tumor tissue. Immunofluorescence staining highlights immunoreactivity for the intermediate filament nestin (d, red) and the calcium binding protein S100β (e, red), while immunoperoxidase staining shows nuclear immunoreactivity for the transcription factors Sox10 (g, brown) and Sox2 (h, brown). Non-immune rabbit (for S100β, Sox2) and mouse (for Sox10, nestin) IgGs were used as negative control primary antibodies (f and i, respectively). Bisbenzimide (“Bis”, blue) was used as a nuclear counterstain in (d)–(f). Hematoxylin was used as a nuclear counterstain in (g) to (i).