Figure 3
The immunoreactivity of the 2XSB sporadic MPNST cell line recapitulates the pattern seen in the parent tumor. (a–h,m–p) Representative immunofluorescence images showing Sox10 (a-d; red), nestin (e–h; red) and S100β (m–p; red) immunoreactivity in 2XSB cells. (i–l,q–t) Representative negative control images from preparations in which the primary antibody was replaced with non-immune mouse IgG or rabbit IgG are presented in (i–l) and (q–t), respectively. To highlight the morphology of these cells, preparations were simultaneously stained with the actin-binding lectin phalloidin (green channel). Bisbenzimide was used as a nuclear counterstain (blue channel).
