Figure 4 | Scientific Reports

Figure 4

From: Age-dependent and region-specific alteration of parvalbumin neurons, perineuronal nets and microglia in the mouse prefrontal cortex and hippocampus following obesogenic diet consumption

Figure 4

HFHS diet-evoked changes to PFC microglia density and morphology. Mice in the adult cohort had a significantly greater (A,B) IBA-1 fluorescence in all three prefrontal subregions. Consumption of the HFHS diet evoked a selective increase (A,B) in IBA-1 density in the PrL cortex in the adult cohort. Mice that consumed HFHS diet across adolescence had proportionally fewer (C,D) ramified ‘resting’ microglia in all three regions and more hypertrophied ‘active’ microglia in the PrL and OFC. In contrast, no diet-related changes (C,E) in PFC microglia morphology was seen in mice that consumed HFHS diet as adults. Data were assessed using two-way ANOVAs and *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Representative photomicrographs were taken at 40X magnification (green—IBA-1 + microglia), scale bars show 50 μm for IBA-1 + density and 10 μm for IBA-1 + morphology.

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