Table 2 Limit of detection of IS6110-qPCR assay.

From: Development of a two-step nucleic acid amplification test for accurate diagnosis of the Mycobacterium tuberculosis complex

DNA copy number

N-RTP (with all primer)b

HS-RTPa (without external primer)c

Average Ct values (± SD)

Interpretation (representation)

Average Ct values (± SD)

Interpretation (representation)

5

27.5 ± 0.6

Positive (20/20)

36.5 ± 0.3

Positive (20/20)

1

30.2 ± 0.8

Positive (17/20)

39.2 ± 0.9

Positive (19/20)

0

ND

Negative

ND

Negative

  1. Ct, Cycle threshold; SD, Standard Deviation; ND, not determined.
  2. aHigh sensitive real-time PCR condition were described by Wang et al. 2019.
  3. bAll primers contain NF6110, NR6110, IS4F, IS4R, IS4P, Ld2F, Ld2R, RPLd.
  4. cExternal primers (NF6110 and NR6110) were replaced by TE buffer.
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