Figure 2
From: Structural insights into phosphatidylethanolamine formation in bacterial membrane biogenesis
Overall crystal structure of apo E. coli PSD. (A) A structure of apo α2β2 heterotetramer. Left and right, two views are related to 90° rotations. The beta chain is illustrated in sky blue, alpha chain in orange, and DDM as stick in white. Pvl, pyruvoyl residue; DDM, dodecyl-beta-d-maltoside. (B) Topology of the αβ-heterodimer. An identical color scheme is used as in (A). (C) A close-up view of the auto-cleaved site. A Fo-Fc omit map for pyruvoyl residue contoured at 3 σ is displayed in mesh. Other residues and DDM were omitted for clarity. Distances between Cα of Pvl-254 and Gly-253 and two interactions in salt bridge designated as dashed lines, which were calculated by averaging values from 6 Apo-PSD protomers. (D). Sliced view of Apo-PSD1 is shown to highlight the funnel-shaped active site. Electrostatic potential is mapped on the surface of the enzyme. Two pyruvoyl residues on the active sites and DDM molecules are shown in sticks.
