Figure 4
From: Structural insights into phosphatidylethanolamine formation in bacterial membrane biogenesis
Phospholipid-bound structures. (A) A scheme of NaCNBH3-dependent reduction of Schiff base. Ptd, phosphatidyl moiety (B) MALDI-ToF analysis of phospholipid conjugation on α-chain. The MS peaks of apo and modified α-chains are marked as gray dashed lines and black arrows for comparison of the m/z values. Observed and calculated m/z values of conjugates are 4925.6, 4926.6 (8:0/8:0 PE-conjugated), 4981.8, 4980.7 (10:0/10:0 PE-conjugated), and 5093.8, 5092.8 (14:0/14:0 PE-conjugated), respectively. (C) A zoomed-in view of linked phospholipid from 8PE-PSD (Left) and 10PE-PSD (Right). A Fo–Fc omit map of the phospholipid is illustrated as mesh contoured at 2.5 σ. Carbon atoms of the lipid are displayed in yellow, oxygen in red, nitrogen in blue, and phosphorous in purple. (D) Overlay of the acyl-chains of bound lipid in the hydrophobic pocket from all four subunits of 10PE-PSD structure. Hydrophobic residues surrounding the acyl-chains are labeled and highlighted as light blue stick.
