Table 2 Summary of NGS data. VH genes of panning samples were analyzed by Miseq. Raw sequence reads were obtained (NGS reads). Correct sequences with antibody structure were extracted using BLAST based software (Total sequences).
Panning round | NGS reads | Total sequences | Unique sequences | Unique/Total |
|---|---|---|---|---|
0 | 890801 | 453896 | 444170 | 0.979 |
1 | 912660 | 789338 | 786040 | 0.996 |
2 | 1227934 | 1083376 | 1051847 | 0.971 |