Figure 6 | Scientific Reports

Figure 6

From: Interleukin-33 and thymic stromal lymphopoietin, but not interleukin-25, are crucial for development of airway eosinophilia induced by chitin

Figure 6

Importance of TSLP and IL-33, but not IL-25, for large-size chitin-induced eosinophilia. Mice were intranasally treated one time with 100 μg of chitin (large size: 70–100 μm) suspended in saline or with saline alone. Twenty-four hours later, BAL fluids and lungs were collected. (a) Numbers of eosinophils and neutrophils in the BAL fluids from C57BL/6N-wild-type (n = 16–26), Tslp−/− (n = 22), Il25−/− (n = 17) and Il33−/− (n = 17) mice. (b) IL-5 levels in the BAL fluids of mice in (a). (c) Detection of TSLP expression in bronchial epithelial cells by IHC. (d) Detection of IL-33 expression in alveolar epithelial cells by IHC. (e) IL-5 and IL-13 levels in the culture supernatants of ILC2s in the presence and absence of TSLP, IL-25 and IL-33. (a,b) Data from 2 independent experiments were pooled and shown as the mean + SE *P < 0.05 vs. wild-type mice. (c,d) Data show a representative result from the lungs of 3 mice. (e) Data show a representative result from 2 independent experiments, which gave similar results.

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