Table 2 Three developed intra-genic markers used to amplify Cd accumulating genes.

From: Validating a segment on chromosome 7 of japonica for establishing low-cadmium accumulating indica rice variety

Gene

Marker

Primer sequence

Expected size (bp) of IRAT129 and 9311 (IRAT129/9311)

Restriction enzyme

Forward (5′-3′)

Reverse (5′-3′)

OsHMA3

HMA3

AATCCATCCAACCAAACCCGGAA

ATGCCCAGCGATCCAAGCTC

280 bp

BsaHI

OsNramp5

Nramp5

TTGTCATGCCGTACGCTT

CACCAAGGCAGAATGCAA

140/144 bp

 

OsNramp1

Nramp1

GGCTGTAGATACCACATCACC

GCTGCCTCAAGAATTATACCTG

761/355 bp

 
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