Figure 3

The data are presented as the mean ± SEM (n = 7). The significance of p < 0.05 is indicated by * indicating a difference between the groups. The asterisk * in H&E technique represents shows rounded acini with polymorphonuclear cells around prostate epithelium and the arrows in toluidine’s blue demonstrate the mast cells in connective tissue of prostate. CT control, HF high-fat diet, HF + Ex high fat and aerobic exercise, HF + FO high fat and fish oil, HF + FO + Ex high fat, fish oil and aerobic exercise, HF + CO high-fat diet and chia oil, HF + CO + Ex high fat, chia oil and exercise group. Graph V: referring to the number of mast cells per mm2; Graph W: referring to the prostatic epithelium; Graph X: referring to prostatic connective tissue; Graph Y: referring to the amount of prostatic lumen. Bar = 20 µm; Resolution = ×40. The Two-Way ANOVA test was used to compare the means, with the Tukey post-test.