Table 4 Sequences of forward and reverse primers used for RT-PCR. Determined by the formula 2(-ΔCq) (Pfaffl, 2001).

From: Supplementation of polyunsaturated fatty acids (PUFAs) and aerobic exercise improve functioning, morphology, and redox balance in prostate obese rats

Gene Primer

Forward primer

Reverse

GSS

F- TATCTCTGCCAGCTTTGGGG

95

R- TCTTGGAAGCTTCGTTGGTCT

NFR2

F- TCCATTCCCGAGTTACAGTGTC

91

R- TCTCTGTCAGTGTGGCCTCT

NOS2

F- AAACAACAGGAACCTACCAGCT

100

R- GACCACTGAATCCTGCCGAT

SOD1

F- GCGTCATTCACTTCGAGCAG

191

R- CCTCTCTTCATCCGCTGGAC

CAT

F- GCGGATTCCTGAGAGAGTGG

188

R- GAGGGTCACGAACTGTGTCA

GAPDH

F- CCATCACCATCTTCCAGGAG

102

R- TCTCCATGGTGGTGAAGACA

  1. GSS Glutathione synthetase, NRF-2 Nuclear factor erythroid 2-related factor-2, NOS-2 Nitric Oxide Synthase-2, SOD-1 Superoxide Dismutase, CAT catalase, GAPDH Glyceraldehyde 3‐phosphate dehydrogenase gene.
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