Figure 2
From: Acetic-acid-induced jasmonate signaling in root enhances drought avoidance in rice
13C-NMR analysis of xylem sap in acetic acid-treated rice plants. (a) 13C-NMR spectra of xylem sap. Two-week-old rice plants were treated with 30Â mM methyl-13C-labeled acetic acid for 7Â h or 2Â days. Xylem sap was collected and analyzed by 13C-NMR. Peaks in spectra observed in 13C-labeled acetic-acid-treated xylem sap were subtracted from those of non-labeled acetic-acid-treated xylem sap and are shown. Chemical shifts of major peaks are indicated in ppm. (b) 2D 13C-HSQC-1H-COSY spectra of xylem sap collected 2Â days after acetic acid treatment. Chemical shifts of major peaks are shown in the right in ppm. (c) Structures of glutamine and glutamic acid. Positions of carbons assigned in (d) are shown. (d) Summary of observed chemical shifts and reference data of glutamine and glutamic acid. Observed chemical shifts were assigned in nmrdb.org (https://www.nmrdb.org/) and nmrshiftdb2 (https://nmrshiftdb.nmr.uni-koeln.de/). Reference data of chemical shifts in 13C- and 1H-NMR of glutamine and glutamic acid in the database are shown. aNo data available.
