Figure 4
From: CD73 facilitates EMT progression and promotes lung metastases in triple-negative breast cancer
Suppression of CD73 inhibits EMT. Representative images of F-actin immunostaining from (A) APCP-treated 4T1 cells, (B) MDA-MB-231 cells and (C) sh-NT and sh-CD73 4T1 cells in normoxia and 1% O2. Quantification of protrusion lengths of (D) APCP-treated 4T1 cells and (E) MDA-MB-231 cells and (F) sh-NT and sh-CD73 4T1 cells, in normoxia and 1% O2. The results are expressed as mean ± SD from 3 independent experiments, n = 25 cells analyzed in each group. E-cadherin and vimentin protein expressions in (G) APCP- or vehicle-treated 4T1 cells, (H) APCP- or vehicle-treated MDA-MB-231 cells and (I) sh-NT and sh-CD73 4T1 cells. The blots were taken from different parts of the same gel. The results are expressed as mean ± SD, n = 3. *P < 0.05 and **P < 0.001 vs representative controls, by a two-tailed Student’s t-test; #P < 0.05; vehicle- vs. APCP-treated cells under 1% O2.
