Figure 5
From: CD73 facilitates EMT progression and promotes lung metastases in triple-negative breast cancer
CD73 suppression inhibits tumor take, growth and lung metastases. (A) sh-NT and sh-CD73 4T1 cell tumor take rates. The numbers indicate formed tumors per 24 tumor inoculations. P-values were analyzed by Chi-Square test. (B) sh-NT and sh-CD73 tumor growth demonstrated as a function of time. (C) Representative images of CD73-positive cells in sh-NT and sh-CD73 tumors. Scale bar 100 µm. (D) Percentage of CD73-positive cells from 4T1 sh-NT and sh-CD73 tumors. (E) Representative images of pHH3-positive cells as an indicator of proliferation in sh-NT and sh-CD73 tumors. Scale bar 200 µm. (F) Percentage of pHH3-positive cells from 4T1 sh-NT and sh-CD73 tumors. Immunohistochemical staining expressions were quantified by QuPath. (G) Representative H & E stainings from the lungs of tumor bearing mice, metastases are indicated with dotted line. Scale bar 500 µm. (H) Numbers of lung metastases from sh-NT and sh-CD73 groups. Data is expressed as mean ± SEM, by a two-tailed Student’s t-test. *P < 0.05; sh-CD73 vs. sh-NT tumors.
