Figure 2

Cycle threshold (ct) values for recovered JEV RNA from Cx. pipiens body (n = 39 and 18), leg/wing (5 and 14) and saliva samples (0 and 14) in both the 20 °C and 25 °C treatments respectively, significant differences between temperature treatments have been marked with a double asterisk. Viral RNA was only recovered from the saliva of mosquitoes in the 25 °C treatment, suggesting that these mosquitoes were competent to transmit JEV.