Figure 4

Variability of midgut infection by JEV in C. pipiens. Positive immunolabeling observed in posterior midgut of infected blood-fed mosquitoes (a–c) and unsuccessful infection (d). There is variability in the number of positive immunostained cells. Some mosquitoes showed high numbers of immunolabeled cells (a), singly immunostained epithelial cells (b, c) and no positive labelling (d). Scale bar: 100 µm.