Figure 4
tRNA fragments gradually increased in plasma with the progression of liver fibrosis in the NAFLD mouse model. After being fed an HC diet for 4 weeks, BALB/c mice were subjected to 0, 2, 4, or 6 weeks of BDL to induce liver fibrosis (n = 5/group). (A) H&E-stained sections and Masson trichrome-stained sections in representative liver samples, 200 × . (B–D) Quantification of the hepatic expression of collagen 1α1, collagen 1α2, αSMA and TGF-β. n = 5, ∗ p < 0.05, ∗ ∗ p < 0.01, Student’s t-test. (F–H) The expression of tRF-Val-CAC-005, tiRNA-His-GTG-001, and tRF-Ala-CGC-006 was tested in plasma by qPCR, n = 5, ∗ p < 0.05, ∗ ∗ p < 0.01, Student’s t-test.
