Figure 4

Viral replication of UV-irradiated SARS-CoV-2 (1000 MOI) virus in in vitro VeroE6 cells. Vero E6 cells were infected with UV-C irradiated SARS-CoV-2 virus at a MOI of 1000. Culture supernatants were harvested at the indicated times (24, 48 and 72 h) and virus titers were measured (A,B) by absolute copy number quantification (Real-Time PCR). Viral replication was assessed even on cell lysate harvested at the end of cell cultures (72 h) (C). All cell culture conditions were seeded in duplicate. Panel (D) reports the plots of the measured virus copies normalized at the untreated sample in the different conditions. For descriptive purposes, mean values and whiskers representing the observed half-ranges are shown.