Figure 4 | Scientific Reports

Figure 4

From: Discovery, characterization and functional improvement of kumamonamide as a novel plant growth inhibitor that disturbs plant microtubules

Figure 4

Analysis of the inhibitory effect on growth of the kumamonamic acid derivative, kumamonamic acid nonyloxy derivative (KAND). (a) Seven-day-old, wild-type Col seedlings were grown on MS plates with the indicated concentrations of KAND 11. Scale bar = 1 cm. (b) Quantification of the root length. Letters indicate significant differences (Tukey’s HSD test, p < 0.05). n > 16. Data are shown as average ± SD. (c) Confocal microscopy of propidium iodide-stained wild-type Col roots grown on MS plates with or without 25 μM KAND 11. White brackets indicate root meristem. Scale bar = 100 μm. (d) Quantification of the root meristem size (n = 10 to 11). Statistical differences were determined by t-test (p < 0.05). Bars indicate mean meristem size. (e) Differential interference contrast (DIC) microscopy of root meristems containing CDKB2;1pro:CDKB2;1-GUS constructs, Five-day-old seedlings grown on MS plates with or without 25 μM KAND were stained and analyzed.

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