Figure 5 | Scientific Reports

Figure 5

From: Discovery, characterization and functional improvement of kumamonamide as a novel plant growth inhibitor that disturbs plant microtubules

Figure 5

Inhibitory effects on growth of the KAND 11 in non-Arabidopsis organisms. (a) Two-week-old, wild-type Nicotiana tabacum SR-1 seedlings were grown on vertically placed MS plates with 25 μM of KAND 11. (b) Two-week-old, wild-type N. tabacum SR-1 seedlings were grown on horizontally placed MS plates with 200 μM of KAND 11. (c) Two-week-old, wild-type Marchantia polymorpha Tak-1 gemmae were grown on Gamborg B5 plates with the indicated concentrations of KAND 11. Red arrows indicate gemmae that ceased growing for the duration of the 2-week incubation. (d) Cell proliferation assay of HeLa cells. The numbers of viable cells were measured using a Cell counting kit-8 (Dojindo) at fixed time intervals. As a control, HeLa cells were treated with 5 μg/ml of actinomycin D (Act D), which inhibits transcription by RNA polymerases and causes cell death. The assays were conducted in triplicate. (e) Cell proliferation assay of Escherichia coli bacteria. Growth of E. coli was analyzed by measuring OD600. As a control, cells were treated with 50 μg/ml of ampicillin (Amp), which inhibits bacterial cell wall synthesis. The assays were conducted in triplicate.

Back to article page