Figure 4
Immunostaining showing the expression of GFAP (astrocytes) in the medial prefrontal cortex (mPFC) and hippocampus CA3, CA1, and dentate gyrus (DG) of mice after 6 weeks of injections with saline (control: a, e, i, m), 15 μg/g nVLDL (b, f, j, n), or 15 μg/g metVLDL (c, g, k, o). Magnification = 20X; scale bar = 50 μm. The percentage of GFAP immunostaining (%) in mPFC (d), CA3 (h), CA1 (l) and DG (p), calculated by using cellSens Dimension software (version 1.13, Olympus), is compared among groups (n ≥ 7 in each group). Data are shown as the mean ± standard error of the mean. *p < 0.05, **p < 0.01, ***p < 0.001 vs. control. #p < 0.05, ###p < 0.001, nVLDL vs. metVLDL groups. P-values were determined by using one-way analysis of variance with the Newman-Keuls post-hoc test.
