Figure 5 | Scientific Reports

Figure 5

From: Utilization of patterned bioprinting for heterogeneous and physiologically representative reconstructed epidermal skin models

Figure 5

Histological examination of skin models reconstructed via manual seeding or bioprinting with shLUC or shFLG cells. (A) H&E sections of shLUC reconstructed samples manually seeded show a well-organized epidermal architecture, while (B) shFLG reconstructed samples demonstrate a pronounced hypogranulosis, consistent with FLG down-regulated skin. These data are consistent with those obtained via bioprinting (C,D). Manually seeded shLUC models expressed high levels of (E) GFP, which is not presented in shFLG samples (F). Manually seeded samples are indistinguishable from their bioprinted counterparts (G,H). Lastly, manually printed shLUC models highly express FLG (I), in contrast to samples reconstructed with shFLG NHK (J). There is no discernible difference in expression in models reconstructed using bioprinting (K,J). Images representative of 8 separate samples. SC stratum corneum, epi epidermis, mbrne polycarbonate membrane support.

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