Table 1 List of target specific primer sequences for real time RT-qPCR analysis used in this circadian study.
Target gene | Length (bp) | Forward primer (5′ → 3′) | Reverse Primer (5′ → 3′) |
|---|---|---|---|
PER2 | 108 | GTATCCATTCATGCTGGGCT | TCGTTTGAACTGCGGTGAC |
PER3 | 146 | TCAGTGTTTGGTGGAAGGAA | TCTGGGTCAGCAGCTCTACA |
CLOCK | 137 | AGTGGATTTGGCTTCAGACT | TTCAATGCCAAGTTCTCGTC |
ARNTL | 123 | TGTGCTAAGGATGGCTGTTC | GCCCTGAGAATGAGGTGTTT |
CRY1 | 102 | CCACGAATCACAAACAGACG | CTCCAATGTGGGCATCAAC |
CRY2 | 145 | TGCAGGTTGTACTCTGCTGC | TGAAGAACTCAGCAAACGGG |
NPAS2 | 136 | GCAAGGTCTTGAAAGGGTGT | TCTTCTCAGAGGCAGCTTGA |
NR1D1 | 128 | GAAGCTGCCATTGGAGTTGT | AAGACATGACGACCCTGGAC |
NR2D2 | 148 | GCCTCCACAGAGTTGACGTT | GAGCAGGGGATCTGCTAAAC |
PPIA | 124 | TCCTTTCTCTCCAGTGCTCAG | CACCGTGTTCTTCGACATTG |
