Figure 5
From: High-intensity leg cycling alters the molecular response to resistance exercise in the arm muscles
The levels of MuRF-1 (A, C) and MAFbx (B, D) mRNA and protein, as well as of PGC-1α1 (E), PGC-1α4 (F) and PGC-1α1 (G) mRNA in muscle biopsies taken at rest and following 90 and 180 min of recovery during both trials. The values presented are means ± SD for 8 subjects. *P < 0.05 vs. Rest, #P < 0.05 vs. the R-Arm trial. Symbols denoted with a line represent a main effect. Symbols without a line represent an interaction effect with corresponding significant differences in the post-hoc analysis. The mRNA levels were normalized to that of GAPDH mRNA and analyzed using the 2−ΔCT procedure. The level of protein was related to the Memcode™ protein stain, with representative bands (from strips of full-length blots) from one subject shown above each graph. The bands have been rearranged, and separated with a white space, to fit the order of the trials in the graphs.
