Figure 1 | Scientific Reports

Figure 1

From: Colonization of distant organs by tumor cells generating circulating homotypic clusters adaptive to fluid shear stress

Figure 1

A limited number of clones dominates CTCs and DTCs. (a) Representative ventral (upper panels) and dorsal (lower panels) bioluminescent images of mice 30 days after orthotopic injection of SAS-GL cells. (b) Representative data for human Alu (hAlu) sequence amplification 30 days after orthotopic injection of SAS-GL cells that indicated SAS-derived DTC presence. Cultured SAS-GL cells (left end) and BM from nontransplanted mice (right end) were controls. mGapdh, mouse Gapdh. Uncropped gels can be found in Supplementary Information. (c) Lentiviral barcode strategy for identifying clonal populations in SAS cells. (d) Unique seeding clones in the primary tumor, CTCs, and BM-DTCs were quantified and plotted. Above the bars are the percentages of unique clones in each organ site and the primary tumor. Values are means ± SEM (n = 5 mice), *p < 0.005; †p < 0.01 (two-tailed t tests). (e) Percentages of dominant clones (top 75%) relative to the total number of seeding clones in each lesion. (f) Percentages of dominant clones (top 75%) in each lesion relative to the total number of unique clones in the primary tumor.

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