Figure 3
From: Anti-mucin 1 chimeric antigen receptor T cells for adoptive T cell therapy of cholangiocarcinoma
Anti-MUC1-CAR constructs, anti-MUC1-CAR T cell generation and their anti-tumor responses. (a) A schematic representation of the anti-MUC1-CAR2 (top) and anti-MUC1-CAR4 (bottom) lentiviral constructs. H represents hinge (spacer) domain. (b) Representative transduction efficiency of the untransduced T cells (UTD, gray), anti-MUC1-CAR2 T cells (red), and anti-MUC1-CAR4 T cells (green) detected by flow cytometry. (c) The transduction efficiency summarized from 4 independent experiments. (d) The bar graph represented percentages of KKU-213A cell lysis from 3 independent experiments. (e) T cell expansion after exposure to either MMNK-1 or KKU-213A cells. All data with error bars represents mean ± SD. Statistical differences were analyzed by Student t-test (asterisks indicate p-values: *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001).
