Figure 6
From: Anti-mucin 1 chimeric antigen receptor T cells for adoptive T cell therapy of cholangiocarcinoma
Cytotoxic function of anti-MUC1-CAR4 T cells on MUC1-expressing CCA cells. (a) The remaining target cells detected by fluorescence microscopic method after co-culturing with anti-MUC1-CAR4 T cells. (b) Specific cell lyses of MMNK-1, KKU-100, and KKU-213A cells after co-culture with the anti-MUC1-CAR4 T cells, examined by luciferase assay at E:T ratios of 1:1, 2.5:1, and 5:1, respectively. Four independent experiments were conducted and summarized (mean ± SEM). (c) The representative cell gating to detect granzyme B in UTD T and anti-MUC1-CAR4 T cells. (d) The bar graphs showed percentages of the granzyme B-producing cells after exposure to MMNK-1, KKU-100, and KKU-213A. The data were summarized from 3 independent experiments (mean ± SD). The statistical differences were analyzed by Student t-test (asterisks represent p-values: *p < 0.05, **p < 0.01, ***p < 0.001).
