Figure 7
From: Anti-mucin 1 chimeric antigen receptor T cells for adoptive T cell therapy of cholangiocarcinoma
Anti-tumor effect of anti-MUC1-CAR4 T cells against MUC1-expressing KKU-213A spheroids. (a) Representative results of KKU-213A spheroids expressing a green fluorescence protein; left panel: no treatment (NT); middle panel: spheroids co-cultured with untransduced T cells (UTD T); right panel: spheroids co-cultured with anti-MUC1-CAR4 T cells (E:T ratio of 10:1). The spheroids were captured under a fluorescence microscope at 10 × objective lens. (b) The bar graphs represent summarized data of corrected total cell fluorescence (CTCF) from 4 independent donors (mean ± SD). The statistical differences were analyzed by Student t-test (asterisks indicate p-values: *p < 0.05, **p < 0.01, ***p < 0.001).
