Figure 3

Dividing nodes using resources by AIBS on gene expression and fiber projection. (a) The four largest nodes in the original AV: CP, Caudoputamen, 26,040 nL; MOB, Main olfactory bulb, 16,406 nL; PIR, Piriform area, 11,591 nL; CA1, Field CA1 in the hippocampus, 10,278 nL. (b) Example of dividing nodes. Leaf nodes in AObase (left) were divided to new leaf nodes using resources at AIBS on gene expression or axonal fiber innervation, obtaining AOdetailed (right). Ellipses and rectangles respectively denote leaf nodes and inner nodes. Filled and open nodes signify nodes with and without corresponding voxels in AV: CA, Ammon’s horn. (c,d) Dividing leaf nodes CA1 and CP based respectively on a gene-expression energy and axonal projection density. Upper panels: coronal sections of volume data obtained at AIBS for gene-expression energy of a gene Wfs-1 (c) or axonal projection-density from the insula cortex to CP (d). Right: histograms of the gene-expression energy within a node CA1 fitted with two Gaussian curves (c) and of the projection-density within a node CP fitted with a Gaussian curve and a Poisson distribution curve (d). Dashed line: an inflection point of the fitted curve used as a threshold. Lower panels: divided nodes. Leaf nodes CA1 and CP were divided, respectively, to two by the thresholds, resulting in nodes “CA1_geneH (red) and CA1_geneL (blue)” and “CP_fiberH (red) and CP_fiberL (blue)”. Axonal projection data were flipped horizontally to obtain symmetrical projection data before thresholding. Values at the lower left represent the anterior–posterior (AP) distance from bregma in millimeters. Scale bar (c,d,f), 5 mm. (e) An icicle plot of anatomical hierarchy of FAAdetailed. Arrows indicate divided leaf nodes based on gene expression in the left (CA1_geneL_L and CA1_geneH_L) and the right (CA1_geneL_R and CA1_geneH_R) side of the brain. Arrowheads show divided leaf nodes based on fiber projection density in the left (CP_fiberL_L and CP_fiberH_L) and the right (CP_fiberL_R and CP_fiberH_R) side of the brain. A zoomable HTML version of this plot is available. (f) Sectional images of AVdetailed shown in random color scheme. Divided nodes for CA1 (arrows) and CP (arrowheads) on both sides of the brain are shown. Specifically, arrows indicate nodes “CA1_geneL_L” (ID 322, blue) and “CA1_geneH_L” (ID 323, green) in the left brain, and nodes “CA1_geneL_R” (ID 1006, yellow) and “CA1_geneH_R” (ID 1007, green) in the right brain. Similarly, arrowheads indicate nodes “CP_fiberL_L” (ID 372, orange) and “CP_fiberH_L” (ID 373, lime) in the left brain, and nodes “CP_fiberL_R” (ID 1056, green) and “CP_fiberH_R” (ID 1057, red) in the right brain.