Figure 4
Changes in beta diversity of the cecal microbiome are associated with high and low expression of biological signatures of inflammation. (A) Principal coordinates ordination plot of weighted UniFrac coordinate analysis of the cecal microbiome was associated with low (pg/ml, pine green, n = 20) and high jejunum occludin concentrations (pg/ml, red, n = 16). Axis 1 explains 37.07%, axis 2 explains 15.93%, and axis 3 explains 10.94% of the variance in the microbiome data analysis such that a total of 62.94% of variance in the dataset is represented here. (B) Principal coordinates ordination plot of unweighted UniFrac analysis of the cecal microbiomes showing separation of samples with low plasma monocyte chemoattractant protein-1 concentrations (MCP-1, pg/ml) (blue-grey, n = 16) and high plasma MCP-1 concentrations (apricot, n = 14). Plasma MCP-1 data were not collected for n = 6 samples (grey). (C) Principal coordinates ordination plot of unweighted UniFrac analysis of the cecal microbiomes showing separation of samples with low (pg/ml, goldenrod, n = 19) and high plasma mucin-2 concentrations (pg/ml, deep purple, n = 15). Mucin-2 data were not collected for n = 2 samples (grey). (B,C) Principal coordinates ordination of unweighted UniFrac: axis 1 explains 17.91%, axis 2 explains 13.43%, and axis 3 explains 8.90% variance in microbiome analysis such that a total of 40.24% of the variance in the dataset is represented here. MCP-1 monocyte chemoattractant protein-1.
