Figure 5

Low soil moisture aggravates the DRR incidence in various chickpea genotypes. Chickpea genotypes PUSA 372, ICC 4958 and JG 62 were used to explore the impact of soil water deficit stress on DRR disease incidence. The experiment was conducted during the rabi season (October to March, 2019–2020). Plants were imposed with LSM, PS, and CS treatments and along with control. Plants were uprooted at the time of maturity and examined for the presence of microsclerotia in the root. Handmade root sections were made at 2 cm from the point of seed attachment. Microsclerotia present on the root was counted. Images (a) show the transverse section of three genotypes in all the treatments and control. Graph (b) shows the number of microsclerotia in the root sections. Images were captured under the 0.5X objective lens of the research stereo microscope SMZ25. Scale bar is 500 µm. N = 3. Significance difference was determined using one-way ANOVA (p < 0.0001) and the asterisk represents the significance. Blue arrows show the microsclerotia. LSM, low soil moisture; PS, pathogen stress; CS, combined low soil moisture and pathogen stress.