Table 2 Evaluation of different sample dilutions and effect on SPR parameters.

From: A surface plasmon resonance based approach for measuring response to pneumococcal vaccine

Sample dilution

Maximum SPR signal*,a

Minimum SPR signal*,b

FC*,c

%CV*,d

1:1

907.40

159.48

5.69

15.40

1:2

706.45

52.40

13.48

8.90

1:4

399.60

33.03

12.10

5.13

1:8

251.51

16.52

15.22

4.20

1:16

146.33

8.26

17.72

3.90

1:32

85.23

4.13

20.64

5.54

  1. *Values in Response Units (RU).
  2. aCorresponding to most anti-PnPs IgGs concentrated sample included in the study, as determined by ELISA (429.13 mg/L).
  3. bCorresponding to less anti-PnPs IgGs concentrated sample included in the study, as determined by ELISA (8.65 mg/L).
  4. cFold Change between the RU values of the maximum and minimum SPR signal determined for each sample dilution.
  5. dMean of the coefficient of variation between replicates for all samples included in the study.
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