Figure 4

Change of immunoreactivity of the 5-HT_2A receptor in the lumbar spinal cord motor neurons. (A) Representative microscopic images of immunoreactivity (IR) of the 5-HT_2A receptor (5-HT_2AR; red) on the lumbar motor neurons of each group are shown. As shown at the top, spinal motor neurons were stained by the anti-ChAT (green) antibody and the IR area of the 5-HT_2AR was confined to the ChAT-stained area (white lines in the binarized image indicate ChAT margins). The nuclei have been stained by 4′,6-diamidino-2-phenylindole (blue). Scale bar: 50 μm. (B) The bar graph with plots presents the quantitative analysis of the IR area of the 5-HT_2AR in the ChAT-positive area of lumbar motor neurons of each group (dashed bar with triangular plots: uninjured group, grey bar with round plots: vehicle-treated group, white bar with rectangular: escitalopram-treated group). ***P < 0.001.