Figure 5

Plasma membrane labeled KCC2 immunostaining on the lumber spinal motor neurons. (A) Representative microscopic images of anti-KCC2 immunostaining (red) on the ChAT positive-lumbar motor neurons (green) of each group are presented. Scale bar: 25 μm. (B) Quantitative analysis of plasma membrane labeled KCC2 of each group, normalized to uninjured and shown as a bar graph (dashed bar with triangular plots: uninjured group, grey bar with round plots: vehicle-treated group, white bar with rectangular: escitalopram-treated group). ***P < 0.001, n.s. non-significant.