Figure 1 | Scientific Reports

Figure 1

From: Hericium erinaceus mycelium and its small bioactive compounds promote oligodendrocyte maturation with an increase in myelin basic protein

Figure 1

Schematic diagram of the experimental designs. (A) The structures of HeA, HeC, and HeS from the H. erinaceus mycelium (HEM) are depicted. (B) In an in vitro experiment, OPCs were seeded in GM for 2 days and then grown in DM for another 2 days. Then, crude HEM, HeA, HeC, or HeS at the indicated concentrations was added to the cultures maintained in DM for 3 consequential days. The cultures were subjected to immunofluorescence to detect GC and MBP, Western blot analysis to measure MBP and PLP, and qPCR to measure OL differentiation-associated genes. (C) The cell markers used to identify OL lineages are illustrated. In this study, immunostaining for NG2, APC, GC, and myelin proteins (MBP and PLP) was conducted to identify OPCs and OLs. Note that the detectable level of MBP and PLP immunoreactivity can be observed in immature OLs in culture.

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