Figure 5 | Scientific Reports

Figure 5

From: Hericium erinaceus mycelium and its small bioactive compounds promote oligodendrocyte maturation with an increase in myelin basic protein

Figure 5

HeA and HeS increased MBP protein production in OL cultures. OPCs were first maintained in DM for 2 days and then exposed to HeA, HeC, or HeS at the indicated concentrations for another 3 days (Fig. 1B). Total proteins were extracted from the cultures and subjected to Western blot analysis to examine MBP (A) and PLP (B) protein levels. The GAPDH level was used as a loading control. The intensity of the immunoreactive bands shown in the immunoblots was quantified by ImageJ software version 1.52a (https://imagej.nih.gov/ij/), and normalized to the level of GAPDH, which was used as a loading control. The results are presented as the means ± SEMs of three repeated experiments. The raw immunoblot images are shown in Figs. S6, S7, and S8. *p < 0.05, **p < 0.01 compared with the control culture.

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