Figure 7

HeA and HeS enhanced the ensheathment of neuronal fibers with MBP⁺-cell processes. As described in Fig. 6A, cerebellar tissue slices were treated with HeA, HeC, or HeS at the indicated concentrations for 7 days. NF200 (green) and MBP (red) double immunofluorescence was conducted. Representative images were captured by confocal microscopy, and the overlap between the MBP signal and NF200⁺-neuronal fibers in 4 random fields per culture was quantified as described in “Materials and methods” section (right panel). The results are presented as the means ± SEMs of three repeated experiments. *p < 0.05, **p < 0.01 compared with the corresponding control culture. Scale bar, 20 μm.