Figure 3
From: Morphofunctional analysis of human pancreatic cancer cell lines in 2- and 3-dimensional cultures

Analysis of cell proliferation ability. (a) Immunocytochemical analysis of Ki-67 in PDAC and HPDE6 cells under 2D (upper row) and 3D (lower row) culture conditions. Scale bar = 50 μm. (b) Quantification of Ki-67-positive cells. Data are shown as the mean ± SD. (c) ATP assay shows that PANC-1, KP4, and MIA PaCa-2 cells in 3D culture had high luminescence signals. Data are shown as the mean ± SD. ATP assay was repeated two times in triplicates.