Figure 1
From: Structure based analysis of KATP channel with a DEND syndrome mutation in murine skeletal muscle
Membrane potential and MgATP sensitivity of Kir6.2/R50P myotubes. (a–c) Mean membrane potential (± SEM) of C2C12 myotube (a), before (white) and after (black) application of glibenclamide (b), and ACh response (c) without infection (N = 5–6), with infection of the Kir6.2/WT AAV vector (N = 5–6), the Kir6.2/R50P AAV vector (N = 5–6). Multiple groups were compared using an ordinary One-Way ANOVA test, followed by Tukey’s test, and two groups were compared using Paired t test. (d) Mean relationship between MgATP and KATP channel conductance (G), expressed as relative to the conductance in the absence of nucleotide (Gc) for non-transfected (n = 4: open circle), WT transfected (n = 4: filled circle) and Kir6.2-R50P transfected (n = 4: closed square). The smooth curves are best fit of the equation shown in methods section to the mean data. For non-transfected cells, IC50 = 0.038 mM, h = 0.58. For WT-transfected cells, IC50 = 0.031 mM, h = 0.58. For Kir6.2-R50P transfected cells, IC50 = 0.09 mM, h = 0.78. The data were analyzed and drawn with Origin Graphing and Data Analysis Software (Version 9; https://www.originlab.com).
